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Image Search Results
Journal: bioRxiv
Article Title: Macrophage-intrinsic MDA5-IRF5 axis drives HIV-1 icRNA-induced inflammatory responses
doi: 10.1101/2024.09.06.611547
Figure Lengend Snippet: THP-1/PMA macrophages were infected with Lailienv GFP/G (MOI 2) in the presence or absence of efaverinz (EFV, 1 µM), raltegravir (RAL, 30 µM), spironolactone (Spiro, 100 nM), and KPT335 (KPT, 1 µM). Cells and culture supernatants harvested at 3 dpi for (A) flow cytometry analysis to measure infection levels (¾GFP+) and (B) ELISA to measure IP-10 secretion. Infection levels (C) and IP-10 secretion (D) in THP-1/PMA macrophages infected with either WT (LailienvGFP/G) or HIV-1/M10 were determined at 3 dpi by flow cytometry and ELISA, respectively. (E) MAVS expression in THP1 cells transduced with shCTRL or shMAVS lentivectors was determined by western blot analy sis. (F, G) LaiiienvGFP/G infected THP-1/PMA macrophages and cell supernatants were harvested 3 dpi for flow cytometry analysis (F) and ELISA (G) to measure infection establishment (¾GFP+) and IP-10 secretion. Data is displayed as the means ± SEM with each dot representing an independent experiment. Statistical significance assessed via 1-way ANOVA with Dunnet’s multiple comparisons test (A-B), unpaired t-test (C-O, F-G). *: p < 0.05; **: p <0.01, ***: p < 0.001, ****: p < 0.0001, ns = not significant.
Article Snippet: In some experiments, THP-1/PMA macrophages were pre-treated (for 20 minutes) with efavirenz (1 μM, NIH AIDS Reagent Program),
Techniques: Infection, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Expressing, Transduction, Western Blot